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Vitamin D Rapid Test
Instructions & Result Interpretation

This page provides step-by-step guidance on how to perform your Vitamin Rapid Test and how to interpret the results.

How to Perform the Test

Vitamin D Instructions.png

Prepare for the Test

  • Wash hands and ensure all materials are at room temperature.

  • Open the test cassette and place it on a flat surface.

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Collect Your Blood Sample

  • Massage your fingertip (middle or ring finger) to increase blood flow.

  • Remove the protective cap from the lancet and position it on the side of your fingertip (avoid the center for comfort).

  • Press firmly until you hear a click. After the puncture, wipe away the first drop of blood with a tissue or gauze

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Transfer the Blood Sample

  • Gently squeeze your fingertip to form a small blood drop.

  • Use the fine-tip dropper to collect blood to fill the thin part of the tube. Ensure to hold the pressure on the fine-tip dropper so that the blood drop remains within the thin part of the tube.

  • Hold the dropper vertically over the test cassette and release 1 drop of blood into the sample well (marked "S").

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Add the Buffer Solution & Start Timer

  • Add 2 drops of the buffer liquid into the sample well.

  • Start a timer and wait 10 minutes for the test to develop.

  • Do not read results after 20 minutes, as they may be inaccurate.

How to Interpret Your Results

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✅ Sufficient Vitamin D (30-100 ng/mL)

  • Three lines appear: C (Control Line), R(Reference) & T (Test Line).

  • The T line is darker than R line on the color chart.

  • Your Vitamin D level is over 30 ng/mL

  • Your Vitamin D levels are in a healthy range
     

⚠ Insufficient Ferritin (Low Iron Levels)​

  • Three lines appear: C (Control Line), R(Reference) & T (Test Line).

  • The T line is lighter than R line on the color chart.

  • Your Vitamin D level is between 10 - 30 ng/mL

  • Consider increasing Vitamin D intake via sunlight, diet, or supplements.
     

❌ Deficient Ferritin (Possible Iron Deficiency)

  • Only Two lines appear: C (Control Line), R(Reference).

  • Your Vitamin D level is lower than 10 ng/mL

  • This indicates Vitamin D deficiency, which may require supplementation.


🚫 Invalid Test Result

  • If no C-line appears, the test is invalid.

  • Retake the test with a new kit.

Important Notes 

⚠ This test provides an indication of Vitamin D levels but is not a medical diagnosis. Always consult a healthcare professional for further advice.

⚠ If your Vitamin D levels are low, you may need to increase sun exposure, eat Vitamin D-rich foods (e.g., oily fish, fortified dairy, eggs), or take supplements.

⚠ Do not reuse any test components. Dispose of used items safely according to local regulations.

⚠ Test results can be affected by extreme temperatures or improper sample collection.

FAQ's

1. What does this test measure?
This test detects 25-hydroxyvitamin D (25(OH)D) in blood, the main indicator of Vitamin D levels.

2. How accurate is the test?
The Vitamin D Rapid Test has 93.88% sensitivity and 98.85% specificity, making it highly reliable.

3. What should I do if my Vitamin D levels are low?
Consider increasing sun exposure, eating Vitamin D-rich foods, or taking supplements. Consult your doctor for further guidance.

4. Can this test be used to monitor Vitamin D supplementation?
Yes! Regular testing can help track how well your body absorbs Vitamin D from food, sunlight, or supplements.

5. What should I do if my test is invalid?
Repeat the test with a new kit and ensure proper sample collection. If issues persist, contact customer support.

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